Transcription-factor binding

DAP-seq

Connect a transcription factor to the genomic DNA sequences it can bind in vitro. DAP-seq can identify binding sites and sequence preferences to prioritize regulatory hypotheses.

Service workflow diagram
A clearer view of your biology

The questions
you can explore.

DAP-seq is an in-vitro DNA-affinity assay. It identifies binding potential and does not by itself establish binding or regulation inside a living cell.

  1. 01Which genomic regions can my transcription factor bind?
  2. 02What sequence motifs are enriched among those binding sites?
  3. 03Which genes are plausible downstream regulatory targets?
Your project outputs

Define what you need
for your next step.

Agreed project scope can include the following. Deliverables and analysis depth are specified during project design.

01

Binding-site maps

Genome-wide candidate binding regions for the transcription factor under the agreed workflow.

02

Motif discovery

Sequence motifs enriched among candidate binding sites.

03

Candidate targets

Nearby genes or regulatory features that can be prioritized for follow-up.

04

Comparative interpretation

Comparison among transcription factors, alleles, or sequence contexts when supported by the study design.

Bring us your sample and question.

We’ll help define the appropriate assay, controls, sequencing, and analysis scope.

Discuss this service